10027/20626
GN Pandey
GN
Pandey
X Ren
X
Ren
HS Rizavi
HS
Rizavi
H Zhang
H
Zhang
Abnormal gene expression of proinflammatory cytokines and their receptors in the lymphocytes of patients with bipolar disorder.
University of Illinois at Chicago
2016
biomarkers
proinflammatory
cytokines
cytokine receptors
bipolar illness
lymphocytes
IL-1 receptors
IL-6 receptors
TNF-α receptors
gene expression
mRNA
2016-05-12 00:00:00
Journal contribution
https://indigo.uic.edu/articles/journal_contribution/Abnormal_gene_expression_of_proinflammatory_cytokines_and_their_receptors_in_the_lymphocytes_of_patients_with_bipolar_disorder_/10757585
Objectives: Abnormalities of protein levels of proinflammatory cytokines and their soluble
receptors have been reported in plasma of bipolar (BP) patients. In this study we tested the
hypothesis that the mRNA expression of membrane-bound receptors for proinflammatory
cytokines will be altered in the lymphocytes of BP patients.
Methods: We determined protein and mRNA expression of proinflammatory cytokines, and
mRNA expression of their receptors in the lymphocytes from 29 drug-free, hospitalized BP
patients and 30 drug-free normal control subjects. The subjects were diagnosed according to
DSM-IV criteria. Plasma protein levels of cytokines were determined by ELISA; mRNA levels in
lymphocytes were determined by the qPCR method.
Results: We found that mean mRNA levels of proinflammatory cytokines IL-1β, IL-6, TNF-α,
their receptors, TNFR1, IL-1R1, and the antagonist IL-1RA were significantly higher in the
lymphocytes of BP patients compared with normal controls.
Conclusions: This study suggests that the observed abnormalities of membrane-bound
cytokine receptors may alter the functional response of cytokines in BP illness and that the mRNA levels of these receptors could be a potential biomarker.