Abnormal gene expression.pdf (296.86 kB)
Abnormal gene expression of proinflammatory cytokines and their receptors in the lymphocytes of patients with bipolar disorder.
journal contribution
posted on 2016-05-12, 00:00 authored by GN Pandey, X Ren, HS Rizavi, H ZhangObjectives: Abnormalities of protein levels of proinflammatory cytokines and their soluble
receptors have been reported in plasma of bipolar (BP) patients. In this study we tested the
hypothesis that the mRNA expression of membrane-bound receptors for proinflammatory
cytokines will be altered in the lymphocytes of BP patients.
Methods: We determined protein and mRNA expression of proinflammatory cytokines, and
mRNA expression of their receptors in the lymphocytes from 29 drug-free, hospitalized BP
patients and 30 drug-free normal control subjects. The subjects were diagnosed according to
DSM-IV criteria. Plasma protein levels of cytokines were determined by ELISA; mRNA levels in
lymphocytes were determined by the qPCR method.
Results: We found that mean mRNA levels of proinflammatory cytokines IL-1β, IL-6, TNF-α,
their receptors, TNFR1, IL-1R1, and the antagonist IL-1RA were significantly higher in the
lymphocytes of BP patients compared with normal controls.
Conclusions: This study suggests that the observed abnormalities of membrane-bound
cytokine receptors may alter the functional response of cytokines in BP illness and that the mRNA levels of these receptors could be a potential biomarker.
Funding
This research was supported by a grant RO1-MH-56528 (Dr. Pandey) from the National Institute of Mental Health, Rockville, MD.
History
Publisher Statement
This is the pre-peer reviewed version of the following article: Pandey, G. N., Ren, X. G., Rizavi, H. S. and Zhang, H. Abnormal gene expression of proinflammatory cytokines and their receptors in the lymphocytes of patients with bipolar disorder. Bipolar Disorders. 2015. 17(6): 636-644. 10.1111/bdi.12320.Publisher
Wileyissn
1398-5647Issue date
2015-09-01Usage metrics
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